A cost-effective and highly integrated imaging analysis system with intelligent image acquisition and analysis capabilities.

Main features:

IntegratedMolecular Devices has over 30 years of experience in automatic imaging and new products, ImageXpress Pico is a highly integrated closed, desktop level, multifunctional imaging analysis system; It can be easily installed in any laboratory, and you can also choose the most suitable objective lens, filter, and whether to add an environmental control system according to your experimental needs.

Environmental control module: software real-time temperature controlPerform live cell experiments under optimal cellular conditions for CO ₂, O ₂, and humidity.

Z-Stack imaging mode: produces clear images with greater depth of field. Obtain a series of images with different focal points to capture more details than a single slice. You can use all the layers or choose the corresponding layer as the final projection.moreLEDs and filter modules - Cy5 TRITC、FITC、DAPI、Texas Red、CFP、 White light and RGB, there is always a channel that suits your experimental needs.

The software has added a dual label expression analysis module, which can achieve multi wavelength cell classification and meet the requirements of multi parameter cell analysis experiments. Such as multi parameter cytotoxicity, cell viability (nuclear staining), stem cell differentiation, etc.

Instrument parameters

Operation mode: White light/bright field, RGB color imaging, fluorescence, navigation positioning shooting (glass slide)
Objective lens: 6-digit interchangeable objective lens converter.
Leica microscope objective FLUROTAR: 4x/NA 0.13, 10x/NA 0.32, 20x/NA 0.40, 40x/NA 0.60, 63x/NA 0.70
Channels: Cy5, TRITC, FITC, DAPI, Texas Red, CFP White and RGB
Automatic focusing method: hardware automatic focusing or hardware assisted image automatic focusing
Imaging methods include monochrome, multi-color, time series Z-stacking
Supporting samples: 1- to 384 well plates and slides
Operating System:Windows 10 (server), Windows 10, and Mac OS (client)
Environmental control attachments:

Room temperature -40 ° C

CO2: 0.1%‒20%

O2: 1‒19%

Humidity control to minimize evaporation to the greatest extent possible

Dimensions (cm): 45.3 (H) x 55.1 (W) x 43.5 (D)

Weight (kg): 38 kg including accessories

application area

It can easily achieve imaging and biological analysis of low-density cells and tissues in well plates, including angiogenesis, apoptosis, autophagy, cell counting, label free cell counting, cell differentiation, endocytosis, mitotic index, lysosome detection, mitochondrial detection, neural process tracking, protein expression index, viral infection, etc.

Experimental examples

Mitochondrial membrane potential detection

Mitochondrial function, as a key indicator for evaluating cellular health, can obtain corresponding data by detecting changes in its membrane potential. The depolarization of mitochondrial membrane potential is an important early signal for hypoxia injury or oxidative stress response, and cationic fluorescent dyes are effective tools for evaluating mitochondrial membrane potential.

Utilization after compound treatmentMitoTracker Orange and nuclear dye molecules were used for staining, combined with ImageXpress Pico personal high connotation imaging analysis system and CellReporterXpress software. By utilizing pre-set modules, the integrity and potential changes of mitochondrial membrane can be comprehensively and effectively evaluated.

PC12 cells (ATCC) were pre seeded at a density of 6000 cells per well in a black bottomed 384 well plate and incubated for 48 hours. The autophagy effect was evaluated by adding various compounds for 24-48 hours, and then the effect was detected by live cell staining using Cyto ID autophagy detection kit (ENO Life Sciences). MitoTracker Orange dye was used to detect mitochondrial health, and Hoechst recognized the nucleus (at concentrations of 0.2 μ M, 0.2 μ M, and 1 μ M, ThermoFisher, respectively). The ImageXpress Pico cell imaging system obtains images using 20x or 40x objective lenses and different fluorescent channels required by dyes (FITC, TRITC, and DAPI, respectively). The analysis of image results is carried out using the particle size application module within the CellReporterXpress software. Particle size logic detection methods and small target feature evaluation, such as autophagy, mitochondrial count in cells, and precise segmentation of cells using nuclear dye molecules. The obtained parameters include the number of particles and the total area. Top: Treat cells with 30 μ M chloroquine to obtain images and analysis results. Bottom: Results of MitoTracker Orange treatment on cells in the image and pseudocolor analysis quality control group. The nucleus is blue, mitochondria are red, and autophagosomes are green.

Neurotoxicity Research

The nervous system is highly sensitive to many toxic compounds and harmful substances generated in the natural environment. During the occurrence of diseases, neurotoxicity can cause temporary or sustained damage to the brain or peripheral nervous system, such as spinal cord injury, stroke, traumatic brain injury, etc. At the same time, this neurotoxicity is also a major cause of many neurodegenerative diseases such as Alzheimer's and Parkinson's.

High throughput analysis is a quantitative analysis method that can measure the length of neural synapses to characterize the positive or negative effects of the analyte. The neural network isAfter ß - tubulin labeling, the ImageXpress Pico personal high content imaging analysis system can be used to obtain images and visualize neural networks. The Neurite Outgrowth module in CellReporterXpress image processing software can easily obtain experimental parameters such as synaptic length.

By markingAfter imaging with the dye molecule (green) of b-tubulin, the differences in cells between the quality control group and the compound treatment group were observed through software tracking and analysis. After inducing the differentiation of multifunctional stem cells into neural cells and treating them with compounds for 3 days, AF488 linked anti - β - tubulin (TUJ-1) antibody (1:100 BD Biosciences) was used for fixed staining. ImageXpress Pico automatic cell imaging system's 10x Plan Fluor objective and FITC and DAPI channels were used for image acquisition. After image acquisition, the neural axon growth tracking analysis algorithm was used for analysis. The green shaded pseudocolor on the right showed growth status, the blue shaded pseudocolor showed cell nucleus, and the branch nodes were displayed in pink. The neural network fracture detection method detects neuronal changes by adding 1 μ M indicator compound. The EC50 value is evaluated by reducing the number of detection branches after adding 2.8 μ M rotenone and 0.071 μ M methyl mercury.

aboutMolecular Devices

Molecular Devices was founded in Silicon Valley, USA in the 1980s. As an excellent brand of high-throughput instruments and equipment worldwide, it has been committed to providing advanced solutions for life science research and drug development. Its products cover microplate detection and analysis, high-throughput screening, high content imaging, efficient cloning screening, and more. The company is renowned in the industry for its continuous innovation, fast and efficient production, high-quality products, and comprehensive after-sales service. Molecular Devices provides you with high-performance analytical detection systems to accelerate and improve drug development and basic life science research. Our products are not only used in scientific research institutions and departments, but also help pharmaceutical and biotechnology companies understand various biological functions at the molecular, cellular, and system levels, and research and develop new treatment methods.